Abstract: The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line – both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines.

RESUMO Os odontomas são considerados os tipos mais comuns de tumores odontogênicos e frequentemente diagnosticados na segunda década de vida. Possuem crescimento lento, comportamento benigno e assintomático, favorecendo sua permanência intraóssea por muito tempo até demonstrarem algum sinal clínico ou serem detectados em radiografias de rotina. O objetivo deste relato foi apresentar um caso clínico de um paciente de 13 anos de idade, sexo masculino, que apresentou atraso na esfoliação do canino decíduo inferior direito. No exame radiográfico, verificou-se uma imagem radiopaca na região anterior da mandíbula, produzida por estruturas que lembravam a formação de dentes, circundada por uma área radiolúcida. Adjacente à lesão, na parte basal, identificou-se a presença do canino permanente inferior direito incluso, apresentando desvio do seu eixo de erupção. O diagnóstico clínico foi definido como odontoma composto. O tratamento consistiu na remoção da lesão e erupção do dente incluso associado, por meio de intervenções cirúrgicas, sem tração ortodôntica, com três anos de acompanhamento e resultados positivos. Existe a possibilidade de erupção cirurgicamente guiada de dente retido, com desvio do seu eixo de erupção e associado à odontoma, através da remoção da lesão e da barreira física (ostectomia) sem tração ortodôntica.

ABSTRACT Odontoma is the most common type of odontogenic tumor and is most often diagnosed in the second decade of life. This benign tumor has a slow growth and is asymptomatic, which favors its persistence in the bone until some clinical sign emerges or the tumor or be detected in a routine radiograph. The aim of this case report was to present a clinic case of a 13-year-old male, who presented delayed exfoliation of the mandibular right primary canine. The radiographic exam revealed a radiopaque image in the anterior region of the mandible produced by structures that resembled the formation of teeth surrounded by a radiolucent area. Adjacent to the tumor, the impacted mandibular right canine was identified with deviation of its eruption axis. The clinical diagnosis was compound odontoma. Treatment consisted of the removal of the tumor and the surgically-assisted eruption of the tooth without orthodontic traction. After three years of follow up, the patient has exhibited positive results. It is possible to surgically assist the eruption of an impacted tooth, with a deviated axis, associated with an odontoma through the removal of the tumor and of the physical barrier (ostectomy) without orthodontic traction.